Agrobacterium-mediated transformation of quaking aspen (Populus tremuloides) and regeneration of transgenic plants.
Identifieur interne : 004B55 ( Main/Exploration ); précédent : 004B54; suivant : 004B56Agrobacterium-mediated transformation of quaking aspen (Populus tremuloides) and regeneration of transgenic plants.
Auteurs : C J Tsai [États-Unis] ; G K Podila ; V L ChiangSource :
- Plant cell reports [ 0721-7714 ] ; 1994.
Abstract
Agrobacterium-mediated gene transformation of Populus tremuloides Michx was accomplished by co-cultivation of leaf disks excised from greenhouse plants with Agrobacterium tumefaciens containing a binary Ti-plasmid vector harboring chimeric neomycin phosphotransferase (NPT II) and ß-glucuronidase (GUS) genes. Shoot regeneration in the presence of kanamycin was achieved when thidiazuron (TDZ) was used as a plant growth regulator. Transformation was verified by amplification of NPT II and GUS gene fragments from genomic DNA of transgenic plants with polymerase chain reaction (PCR) and integration of these genes into nuclear genome of transgenic plants was confirmed by genomic Southern hybridization analysis. Histochemical assay revealed the expression of GUS gene in leaf, stem and root tissues of transgenic plants, further confirming the integration and expression of T-DNA in these plants. This protocol allows effective transformation and regeneration of quaking aspen using greenhouse-grown materials as an explant source. Whole plant regeneration from cuttings of fieldgrown mature quaking aspen and hybrid poplar (P. alba x P. grandidentata) was also readily achieved by using this protocol, which represents a potential system for producing transgenic quaking aspen and hybrid poplar of valuable genotypes.
DOI: 10.1007/BF00233768
PubMed: 24192872
Affiliations:
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Shoot regeneration in the presence of kanamycin was achieved when thidiazuron (TDZ) was used as a plant growth regulator. Transformation was verified by amplification of NPT II and GUS gene fragments from genomic DNA of transgenic plants with polymerase chain reaction (PCR) and integration of these genes into nuclear genome of transgenic plants was confirmed by genomic Southern hybridization analysis. Histochemical assay revealed the expression of GUS gene in leaf, stem and root tissues of transgenic plants, further confirming the integration and expression of T-DNA in these plants. This protocol allows effective transformation and regeneration of quaking aspen using greenhouse-grown materials as an explant source. Whole plant regeneration from cuttings of fieldgrown mature quaking aspen and hybrid poplar (P. alba x P. grandidentata) was also readily achieved by using this protocol, which represents a potential system for producing transgenic quaking aspen and hybrid poplar of valuable genotypes. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24192872</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>14</Volume><Issue>2-3</Issue><PubDate><Year>1994</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Agrobacterium-mediated transformation of quaking aspen (Populus tremuloides) and regeneration of transgenic plants.</ArticleTitle><Pagination><MedlinePgn>94-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF00233768</ELocationID><Abstract><AbstractText>Agrobacterium-mediated gene transformation of Populus tremuloides Michx was accomplished by co-cultivation of leaf disks excised from greenhouse plants with Agrobacterium tumefaciens containing a binary Ti-plasmid vector harboring chimeric neomycin phosphotransferase (NPT II) and ß-glucuronidase (GUS) genes. Shoot regeneration in the presence of kanamycin was achieved when thidiazuron (TDZ) was used as a plant growth regulator. Transformation was verified by amplification of NPT II and GUS gene fragments from genomic DNA of transgenic plants with polymerase chain reaction (PCR) and integration of these genes into nuclear genome of transgenic plants was confirmed by genomic Southern hybridization analysis. Histochemical assay revealed the expression of GUS gene in leaf, stem and root tissues of transgenic plants, further confirming the integration and expression of T-DNA in these plants. This protocol allows effective transformation and regeneration of quaking aspen using greenhouse-grown materials as an explant source. Whole plant regeneration from cuttings of fieldgrown mature quaking aspen and hybrid poplar (P. alba x P. grandidentata) was also readily achieved by using this protocol, which represents a potential system for producing transgenic quaking aspen and hybrid poplar of valuable genotypes. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tsai</LastName><ForeName>C J</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>School of Forestry and Wood Products, Michigan Technological University, 49931, Houghton, Michigan, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Podila</LastName><ForeName>G K</ForeName><Initials>GK</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>V L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1993</Year><Month>11</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>1994</Year><Month>06</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1994</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1994</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24192872</ArticleId><ArticleId IdType="doi">10.1007/BF00233768</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 1992 Jan;98(1):114-20</Citation><ArticleIdList><ArticleId IdType="pubmed">16668600</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1990 Jan;8(9):512-6</Citation><ArticleIdList><ArticleId IdType="pubmed">24226275</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 Jul;93(3):1110-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16667565</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1978 Jul 11;163(2):181-7</Citation><ArticleIdList><ArticleId IdType="pubmed">355847</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1991 Sep;17(3):441-52</Citation><ArticleIdList><ArticleId IdType="pubmed">1653060</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1993 Apr;12(6):303-6</Citation><ArticleIdList><ArticleId IdType="pubmed">24197252</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1991 Dec;17(6):1203-15</Citation><ArticleIdList><ArticleId IdType="pubmed">1932694</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1992 Apr;11(3):137-41</Citation><ArticleIdList><ArticleId IdType="pubmed">24213546</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1986 Dec;5(6):464-7</Citation><ArticleIdList><ArticleId IdType="pubmed">24248407</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1991 Mar 25;19(6):1349</Citation><ArticleIdList><ArticleId IdType="pubmed">2030957</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><noCountry><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name><name sortKey="Podila, G K" sort="Podila, G K" uniqKey="Podila G" first="G K" last="Podila">G K Podila</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Tsai, C J" sort="Tsai, C J" uniqKey="Tsai C" first="C J" last="Tsai">C J Tsai</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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